折腾这么多都是白瞎,STAR就有输出没有别对上的pair-end reads的功能 参见:How To Filter Mapped Reads With Samtools I had the same issue but with Paired End Reads, and I solved using samtools and bamToFastq. You can find bamToFastq here: https://code.google.com/p/hydra-sv/ If you