R中seurat等问题学习

1.Seurat

转自：https://cloud.tencent.com/developer/article/1055892

# Initialize the Seurat object with the raw (non-normalized data).  Keep all
# genes expressed in >=  cells (~0.1% of the data). Keep all cells with at
# least  detected genes
pbmc <- CreateSeuratObject(raw.data = pbmc.data, min.cells = , min.genes = ,
    project = "10X_PBMC")

这里的min.genes是对细胞来说的，过滤掉那些检测到基因个数少于200的细胞。保留>=200的吧。

min.cells=200，包含那些基因，至少在200个细胞里表达了。