转录组

测量单cell，可以认为是一种细胞。细胞株也认为来自同一个细胞。

使用两种方法，找mRNA（polyA）及rmRNA（ribo-minus），然后取交集。

转录组受实验影响，比如小片段没得到。

干细胞与诱导的干细胞序列相似的原因尚不清楚。

转录因子参与的转录过程，相同转录因子，老鼠可以转向干细胞，但是牛就不可以。说明真核细胞转录复杂性。

平衡与跌倒

干细胞的所有基因都在低表达状态。在分化过程中，受到能量分配影响，转录扩增细胞同时关闭一些gene和增多一些gene。HK维持表达，TS会关闭一些。继而形成differentiated cell，然后形成regulation 后的differentiated cell，直到terminally differentiated cell。

通过切片反应Genetic heterogeneity异质性

 

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