fastq,sam文件一些小结（持续补充。。。）

ST-E00211::H5L3NCCXY::::        chr14            141M    =        -    ACTTCACCTCCTGGAGTCCTGGACTTCCCCACATCTCCCCTGCCCCTCCCACGTTTCCATAGTCCAAGGGCCAGAGTAAATGAAAATACAGCAGCCGCCCAAGCAATGGGGCCCATGCTGGGGCTTCAGTCATCCCCATGT    JFJFJFJJJJJJJJJJJJJJJFAJJJJJJJJJFFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFFFAA    AS:i:-    XN:i:    XM:i:    XO:i:    XG:i:    NM:i:    MD:Z:0T0T132A0G3C1    YS:i:-    YT:Z:CP
ST-E00211::H5L3NCCXY::::        chr14            141M    =        -    ACTTCACCTCCTGGAGTCCTGGACTTCCCCACATCTCCCCTGCCCCTCCCACGTTTCCATAGTCCAAGGGCCAGAGTAAATGAAAATACAGCAGCCGCCCAAGCAATGGGGCCCATGCTGGGGCTTCAGTCATCCCCATGT    A<FFFFFFFJJFJFJJ<<JJJJJJJJJJJJFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFJJJJJJFJJJ<JFJJJJJJJJAJJFJJJJJJJJJJJJJJJJJFJJJJFJJJJJJJJJJAJJJJJJJJJJJJJJJJ    AS:i:-    XN:i:    XM:i:    XO:i:    XG:i:    NM:i:    MD:Z:0T0T132A0G3C1    YS:i:-    YT:Z:CP

这是双端测序的sam文件随意挑出的一条测序片段。（sam文件会把同一片段的两条read都保留，从第二列可以知道是read1还是read2），可以发现这条片段已经被测通（因为染色体坐标一致），基因序列整体是相同的，说明read1和read2都是按5‘-->3’的顺序排列的