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RNA测序的质量控制 发表评论 3,112 A+ 所属分类:Transcriptomics   收  藏 ENCODE项目向我们揭示,人类基因组中超过70%能得到转录,只不过不会发生在同一个细胞里.为了研究如此多样的转录本,研究人员开发了许多技术,其中RNA测序(RNA-seq)是最全面也最有效的. 许多人相信,未来基因组数据将会对患者的治疗产生重要的影响.不过也有不少专家质疑基因组分析的准确性和可靠性.为此,美国FDA牵头了RNA测序质量控制(SEQC)项目,评估了多个试验室RNA-seq数据…
Evolutionary approaches towards AI: past, present, and future 2019-10-06 07:28:13 This blog is from: https://towardsdatascience.com/evolutionary-approaches-towards-ai-past-present-and-future-b23ccb424e98 Table of Contents Introduction Genetics and na…
Spike-in Control:添加/加入(某种物质)的对照(组)在某些情况下,待检验样本中不含待测物质或者含有但是浓度很低,为了证明自己建立的方法能对样本中待测物质进行有效的检测,可在待检样本中加入一定量的待测物质(外标)来进行该方法检测能力的验证.有时,这种加入外标的物质,也可用作为阳性对照.请楼主结合具体的语言环境来确定准确的意思.以下是对楼下战友提供的wiki中关于RNA Spike-in 解释部分的翻译,不妥之处,敬请指正:An RNA spike-in is an RNA tran…
MicroRNA in Control of Gene Expression:An Overview of Nuclear Functions微RNA控制基因表达:核功能概述 抽象:小的非编码RNA(ncRNA)能够以序列特异性方式控制基因表达的发现对生物学产生了巨大影响.最近的改进很高吞吐量排序和计算预测方法已经允许发现和几种类型的ncRNA的分类.基于它们的前体结构,生物发生途径和作用模式,ncRNA被分类为小干扰RNA(siRNAs),microRNAs(miRNA),PIWI相互作用的R…
Part 1 <06/05/07 12:08pm> Manao | he is negating a float by printing it, adding a "-" to the string, and converting it back to float <06/05/07 12:08pm> Manao | in the process, of course, he doesn't free the allocated string <06/05…
ERCC是啥? 外部RNA控制联盟,就是一套RNA-seq,基因表达检测过程中的控制系统,使得结果具有可重复性. RNA Spike-in Controls for Gene Expression While early gene expression measurements with DNA microarrays were groundbreaking in their ability to reveal biological activity, the results were irre…
转自:https://blog.cloudflare.com/the-story-of-one-latency-spike/ A customer reported an unusual problem with our CloudFlare CDN: our servers were responding to some HTTP requests slowly. Extremely slowly. 30 seconds slowly. This happened very rarely an…
资源: sci-hub paper CellBench package - github CellBench_data - code for the paper 现在单细胞领域的突出问题就是工具过多,但缺乏gold-standard benchmark datasets,没有一定的标准来衡量工具的好坏. 另外人们也不容易根据自己的问题来选择合适的工具,所以保险起见,现在大家都只用最有名气的那几个工具:seurat.monocle.SC3等. 算法是无穷的,随便套几个方法就能组成一个分析方法,做生…
Dr. Andrew Paul HutchinsDepartment of BiologySouthern University of Science and Technology, Shenzhen, China Every cell contains all of the DNA to encode the plethora of cell types that make up the cells and tissues of the organism. Yet, the vast majo…
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