折腾这么多都是白瞎,STAR就有输出没有别对上的pair-end reads的功能 参见:How To Filter Mapped Reads With Samtools I had the same issue but with Paired End Reads, and I solved using samtools and bamToFastq. You can find bamToFastq here: https://code.google.com/p/hydra-sv/ If you…
~~和唐老师一个实验室的人开发的~~ CD-HIT is a very widely used program for clustering and comparing protein or nucleotide sequences. CD-HIT was originally developed by Dr. Weizhong Liat Dr. Adam Godzik's Lab at the Burnham Institute (now Sanford-Burnham Medical Res…
Directional RNA-seq data -which parameters to choose? REF: https://chipster.csc.fi/manual/library-type-summary.html Directional RNA-seq methods are gaining popularity. Several protocols and products are available for the library preparation step, and…
A survey of best practices for RNA-seq data analysis RNA-seq数据分析指南 内容 前言 各位同学/老师,大家好,现在由我给大家讲讲我的文献阅读报告! A survey of best practices for RNA-seq data analysis ,我把它叫做RNA-seq数据分析指南.这篇文章是由佛罗里达大学等单位的研究人员在1月26日发表在Genome Biology上的,该期刊的影响因子有10.8分.这是这篇文章的通讯作者,…
参考:https://www.biostars.org/p/106291/#106344 1.inner mate distance : the distance from the right most end of the left read to the left most end of the right read 2.the "insert size"=sequence between adapters (actually encompasses R1 and R2 as we…
多表通用的SQL存储过程分页 案例一: USE [Community] GO /****** Object: StoredProcedure [dbo].[Common_PageList] Script Date: 2016/3/8 13:44:58 ******/ SET ANSI_NULLS ON GO SET QUOTED_IDENTIFIER ON GO CREATE PROCEDURE [dbo].[Common_PageList] ( ),---表名 @strFld nvarchar…
