string file = @"C:\sample.xlsx";

        if(System.IO.File.Exists(file))
{ Microsoft.Office.Interop.Excel.Application excelApp = new Microsoft.Office.Interop.Excel.Application();
excelApp.Visible = true; //FOR TESTING ONLY
Microsoft.Office.Interop.Excel.Workbook wb = excelApp.Workbooks.Open(file,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing,
Type.Missing); Microsoft.Office.Interop.Excel.Worksheet ws = (Microsoft.Office.Interop.Excel.Worksheet)wb.Sheets[]; //Selects the first sheet
Microsoft.Office.Interop.Excel.Range range = (Microsoft.Office.Interop.Excel.Range)ws.Cells[, ]; //Select cell A1
object cellValue = range.Value2; #region Extract the image
Microsoft.Office.Interop.Excel.Picture pic = (Microsoft.Office.Interop.Excel.Picture)ws.Pictures(); if (pic != null)
{
//This code will detect what the region span of the image was
int startCol = (int)pic.TopLeftCell.Column;
int startRow = (int)pic.TopLeftCell.Row;
int endCol = (int)pic.BottomRightCell.Column;
int endRow = (int)pic.BottomRightCell.Row; pic.CopyPicture(Microsoft.Office.Interop.Excel.XlPictureAppearance.xlScreen, Microsoft.Office.Interop.Excel.XlCopyPictureFormat.xlBitmap);
if (Clipboard.ContainsImage())
{
Image img = Clipboard.GetImage();
this.pictureBox1.Image = img;
}
}
#endregion //Close the workbook
wb.Close(false,Type.Missing,Type.Missing); //Exit Excel
excelApp.Quit();
}

work great for me in my web application with a little change it was not copying image to clipboard

Thread thread = new Thread(() =>
{
foreach (var pic in ws.Pictures())
{
if (pic != null)
{
//This code will detect what the region span of the image was
int startCol = pic.TopLeftCell.Column;
int startRow = pic.TopLeftCell.Row;
int endCol = pic.BottomRightCell.Column;
int endRow = pic.BottomRightCell.Row;
pic.CopyPicture(XlPictureAppearance.xlScreen, XlCopyPictureFormat.xlBitmap);
if (Clipboard.GetDataObject() != null)
{
Image img = Clipboard.GetImage();
}
}
}
});
thread.SetApartmentState(ApartmentState.STA);
//Set the thread to STA
thread.Start();
thread.Join();

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